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KMID : 0361020000430111102
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 11 p.1102 ~ p.1107
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Expression of Antimicrobial Defensin Peptides of the Human Nasal Mucosa
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Kim Jung-Eun
Jeong Young-Joon
Kimg Jeong-Joon
Yoo Chan-Ki
Lee Heung-Man
Lee Sang-Hag
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Abstract
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Background and Objectives: Defensin, an antimicrobial peptide, is an important component of the innate immune response, particulary at mucosal surface. The present study was undertaken to investigate the expression of expression of defensin in inferior turbinate mucosa and nasal polyp.
Materials and Methods: Expression of β-defensin 1, 2 and α-defensin 5, 6 mRNA was investigated by RT-PCR dot blot hybridization. Immunohistochemistry was utilized for detection of α-defensin 1, 2, 3 in tissue section.
Results: β-defensin 1 mRNA was expressed in all tissue samples, with not so significantly different levels of expression. β-defensin 2 mRNA was detected in patients with normal mucosa. Its expression level was significantly higher in nasal polyp than in turbinate mucosa. α-defensin 5, and 6 mRNA were not expressed in any tissue but α-defensin 1, 2, and 3 were detected in all tissue samples obtained from patients with chronic sinusitis.
Conclusion: The results suggest that β-defensin 1 may play a constitutive role in the nasal defense while α-defensin 1, 2, 3 and β-defensin 2 may be induced in response to local infection or infection or inflammation.
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KEYWORD
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Defensin, RT-PCR, Dot blot hybridization, inferior turbinate, Nasal polyp
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